Introduction Protoplast culture is a laboratory technique that involves the isolation of the plant cell from the cell wall. The protoplasts can then be cultured in vitro, allowing for the study of cellular processes, including cell division, differentiation, and regeneration. Protoplast The technique has many applications in plant biotechnology, including the production of new crop varieties, genetic engineering, and the production of plant secondary metabolites. Below we shall look at the importance of protoplast culture in plant regeneration and how it is used to produce new plants. What Are Protoplasts? Protoplasts are cells that have had their cell walls ... Read More

Introduction Handling and sterilization of plant material is an important aspect of any laboratory or plant research facility. Plant material, such as leaves, stems, and seeds, can be contaminated with microbes and other pathogens that can affect the results of research. To ensure the accuracy and reliability of research, it is important to properly handle and sterilize the plant material. In this tutorial, we will discuss various methods of handling and sterilization of plant material. Part 1: Handling of Plant Material Handling of plant material involves the proper collection and preparation of the sample for sterilization. Before collecting plant ... Read More

Embryo Rescue -A Milestone Embryo rescue is a technique that involves the rescue of developing embryos from immature or unpollinated ovules of sexually incompatible plant crosses. It enables the development of hybrid embryos that would otherwise not survive to maturity. Embryo rescue was first introduced in the early 20th century and has since been extensively used in plant breeding, particularly in generating interspecific and intergeneric hybrids. The basis of embryo rescue is the manipulation of the in vitro culture conditions to provide the developing embryo with the necessary nutrients and support for its growth and development. The success of ... Read More